Computational Optical Microscopy

Resonator diagram

System diagram. A spatial heterodyne is produced using a Michelson interferometer. The sample is attached to a mirror at the end of the signal arm, and this mirror is mounted on a 3-dimensional translation stage.

Optical imaging techniques generally require a large refractive index variation to discern different components of semi-transparent objects. For imaging cells, this necessitates staining, which may not be appropriate for all samples. We've shown how to measure semi-transparent sample properties without staining, using sensitive interferometric techniques and computational reconstruction. The approach can resolve index-variation and attenuation and separate their effects from the image of the object at focus.