Computational Optical Microscopy
System diagram. A spatial heterodyne is produced using a Michelson interferometer. The sample is attached to a mirror at the end of the signal arm, and this mirror is mounted on a 3-dimensional translation stage.
Optical imaging techniques generally require a large refractive index variation to discern different components of semi-transparent objects. For imaging cells, this necessitates staining, which may not be appropriate for all samples. We've shown how to measure semi-transparent sample properties without staining, using sensitive interferometric techniques and computational reconstruction. The approach can resolve index-variation and attenuation and separate their effects from the image of the object at focus.
- K. J. Dillon and Y. Fainman, "Computational Confocal Scanning Tomography," in Computational Optical Sensing and Imaging, OSA Technical Digest (CD) (Optical Society of America, 2009), paper JTuC7.